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Part:BBa_I731008

Designed by: Sammy Sambu   Group: iGEM07_Harvard   (2007-10-14)


OmpA scaffold for loop 1 insertions

This is a scaffold upon which users can insert tags of interest. The point of insertion is the first loop of OmpA.For this purpose, there is an NheI restriction site at which insertions downstream of this point are likely to be exposed to extracellular space in the final protein product.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 136
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 881
    Illegal BamHI site found at 744
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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